"PCR"
454
|
Solexa
|
SOLiD
|
|
Company
|
Roche
|
Illumina
|
ABI
|
Sequencing Method
|
Pyrosequencing
|
Reversible Terminator
|
Supported Oligo Ligation
|
PCR
|
Emulsion PCR
|
Bridge PCR
|
Emulsion PCR
|
Read Length
|
300-400 base
|
36 base
|
|
Error rate
|
~1%
|
>1%
|
~0.1%
|
Time
|
7.5 hours
|
3~5 days
|
8 days
|
Taq DNA Polymerase
|
Pfu Polymerase
|
T4 Polymerase
|
Reverse Transcriptase
|
|
Error rate
|
2.2x10^-5
|
2.6x10^-6
|
1x10^-6
|
10^-3
|
Electrophoresis:
(1) Agarose: Normal DNA
(2) Acerylmide(SDS-PAGE): Protein
(3) Pulse Field: Large DNA
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