DNA Technology

"PCR"

	454	Solexa	SOLiD
Company	Roche	Illumina	ABI
Sequencing Method	Pyrosequencing	Reversible Terminator	Supported Oligo Ligation
PCR	Emulsion PCR	Bridge PCR	Emulsion PCR
Read Length	300-400 base		36 base
Error rate	~1%	>1%	~0.1%
Time	7.5 hours	3~5 days	8 days

	Taq DNA Polymerase	Pfu Polymerase	T4 Polymerase	Reverse Transcriptase
Error rate	2.2x10^-5	2.6x10^-6	1x10^-6	10^-3

Electrophoresis:

(1) Agarose: Normal DNA

(2) Acerylmide(SDS-PAGE): Protein

(3) Pulse Field: Large DNA

Chapter 36 Transport in Vascular Plants

Anthocyanin 花青素 increased under low Pi condition.

Function of Fe:
(1) chlorophyll development
(2) photosynthesis & respiration
(3) breakdown of sugar and complex compounds


"Root"

H position: Epidermis cells where root hair grows.
N position: Epidermis cells without root hairs.

雜論

• 蘋果褐化→蘋果多酚氧化酶。Arctic apple，不會褐化。

農業生物科技

除草劑 Roundup Ready

主成分：Glyphorite
LD50約略和NaCl相當



蘇力菌 Bacillus Thuringiensis bacteria (BT)

主要噴灑在玉米上，抗蟲。但影響到附近的Monarch Butterflies

Chapter 40 The Animal Body

Chapter 43 Animal Transport System

封口機

Chapter 50 Sensation and Movement in Animals

Meissner's corpuscles: 梅思勒小體
Free nerve endings: 游離神經末梢
Pacinian corpuscles: 巴齊尼氏小體

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